Specific Detection of His-Tagged Proteins with Recombinant Anti-His Tag scFv-Phosphatase or scFv-Phage Fusions

Using a cell-bound immunogen, we have generated a monoclonal antibody, 3D5, that recognizes carboxy-terminal oligo-histidine tags (His tags) on a wide variety of proteins. From this monoclonal antibody, we have generated a single-chain fragment of the variable domains (scFv), a dimeric scFv-alkaline...

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Bibliographic Details

Published in:	BioTechniques
Main Authors:	P. Lindner, K. Bauer, A. Krebber, L. Nieba, E. Kremmer, C. Krebber, A. Honegger, B. Klinger, R. Mocikat, A. Plückthun
Format:	Article
Language:	English
Published:	Taylor & Francis Group 1997-01-01
Online Access:	https://www.future-science.com/doi/10.2144/97221rr01