Transformation of CryK13V gene into protoplasts of Trichoderma viride
The lysine in site 13 of cryptogein protein was mutated to valine (K13V) through PCR site-directed mutagenesis. The mutant fragment (CryK13V) was confirmed by enzyme digestion and DNA sequencing. The CryK13V gene was expressed in Trichoderma viride, with constructed vector pCSNTCCm. Transformation w...
| الحاوية / القاعدة: | 浙江大学学报. 农业与生命科学版 |
|---|---|
| المؤلفون الرئيسيون: | , , , |
| التنسيق: | مقال |
| اللغة: | الإنجليزية |
| منشور في: |
Zhejiang University Press
2006-05-01
|
| الموضوعات: | |
| الوصول للمادة أونلاين: | https://www.academax.com/doi/10.3785/1008-9209.2006.03.0270 |
