Demonstrating soft X-ray tomography in the lab for correlative cryogenic biological imaging using X-rays and light microscopy

Abstract Soft X-ray tomography (SXT) enables native-contrast three-dimensional (3D) imaging of fully hydrated, cryogenically preserved biological samples, revealing ultrastructural details without the need for staining, embedding, or sectioning. Traditionally available only at synchrotron facilities...

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Bibliographic Details

Published in:	Scientific Reports
Main Authors:	Stephen O’Connor, David Rogers, Maryna Kobylynska, James A. Geraets, Katja Thaysen, Jacob Marcus Egebjerg, Madeleen C. Brink, Louisa Herbsleb, Michaela Salakova, Leon Fuchs, Frauke Alves, Claus Feldmann, Axel Ekman, Paul Sheridan, William Fyans, Tony McEnroe, Fergal O’Reilly, Kenneth Fahy, Roland A. Fleck, Daniel Wüstner, Jeremy C. Simpson, Andreas Walter, Sergey Kapishnikov
Format:	Article
Language:	English
Published:	Nature Portfolio 2025-11-01
Online Access:	https://doi.org/10.1038/s41598-025-29385-5