Optimization of ultrasound-mediated DNA transfer for bacteria and preservation of frozen competent cells

ABSTRACT The transformation of DNA into cells is the basis of molecular biology. Commonly employed techniques include heat shock transformation, electro-transformation, conjugation, transduction, and protoplast fusion. Recently, ultrasonic transformation technology has been developed to transfer DNA...

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Bibliographic Details

Published in:	Microbiology Spectrum
Main Authors:	Meng Zhang, Rongkang Tang, Fang-Xia Li, Wen-Yu Jin, Jia-Xin Guo, Lin-Zuo Teng, Guangxun Meng, Philippe J. Sansonetti, Yi-Zhou Gao
Format:	Article
Language:	English
Published:	American Society for Microbiology 2024-12-01
Subjects:	ultrasonic plasmid transformation ultrasonic transformation container cryopreservation cryoprotective agents
Online Access:	https://journals.asm.org/doi/10.1128/spectrum.00978-24