CRISPR/Cas9 gRNA activity depends on free energy changes and on the target PAM context
CRISPR/Cas9-mediated cleavage efficiency varies at different target locations. Here the authors explain this variation with binding free energy changes and show that overlapping Cas9 binding sites influence cleavage efficiency by enabling Cas9 sliding.
| Published in: | Nature Communications |
|---|---|
| Main Authors: | , , , , , |
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2022-05-01
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| Online Access: | https://doi.org/10.1038/s41467-022-30515-0 |