Nested PCR amplification of the mitochondrial hypervariable region for non-invasive eDNA detection of Cryptobranchus alleganiensis.

Environmental DNA (eDNA) has proven an invaluable tool in detecting elusive, endangered, or otherwise hard-to-identify species in aquatic ecosystems. The Eastern hellbender salamander (Cryptobranchus alleganiensis) is a fully aquatic amphibian with an increasingly threatened population due to its re...

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Bibliographic Details
Published in:PLoS ONE
Main Authors: Patrick Kurtz, Jorge Santo Domingo, Robert Alexander Pyron, David Wendell
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2025-01-01
Online Access:https://doi.org/10.1371/journal.pone.0328633