Seamless Coupling of Chemical Glycan Release and Labeling for an Accelerated Protein <i>N</i>-Glycan Sample Preparation Workflow

Analytical methods fr direct quantitative <i>N</i>-glycan analysis require a sequence of sample preparation and clean-up steps that result in reduced glycan recovery. Therefore, we aimed to combine glycan release and labeling steps. Based on the hypothesis that the reaction mechanism for...

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Bibliographic Details

Published in:	Bioengineering
Main Authors:	Mumtaz Kasim, Anja Griebel, Grit Sandig, Robert Höltzel, Akshay Malhotra, Stephan Hinderlich, Volker Sandig, Barbara Müller, Hans Henning von Horsten
Format:	Article
Language:	English
Published:	MDPI AG 2023-05-01
Subjects:	glycoproteins glycan analytics <i>N</i>-glycans Hofmann rearrangement glycan sample preparation bioprocessing
Online Access:	https://www.mdpi.com/2306-5354/10/6/651