An adapted protocol to derive microglia from stem cells and its application in the study of CSF1R-related disorders

Abstract Background Induced pluripotent stem cell-derived microglia (iMGL) represent an excellent tool in studying microglial function in health and disease. Yet, since differentiation and survival of iMGL are highly reliant on colony-stimulating factor 1 receptor (CSF1R) signaling, it is difficult...

Full description

Bibliographic Details
Published in:	Molecular Neurodegeneration
Main Authors:	Marie-France Dorion, Diana Casas, Irina Shlaifer, Moein Yaqubi, Peter Fleming, Nathan Karpilovsky, Carol X.-Q. Chen, Michael Nicouleau, Valerio E. C. Piscopo, Emma J. MacDougall, Aeshah Alluli, Taylor M. Goldsmith, Alexandria Schneider, Samuel Dorion, Nathalia Aprahamian, Adam MacDonald, Rhalena A. Thomas, Roy W. R. Dudley, Jeffrey A. Hall, Edward A. Fon, Jack P. Antel, Jo Anne Stratton, Thomas M. Durcan, Roberta La Piana, Luke M. Healy
Format:	Article
Language:	English
Published:	BMC 2024-04-01
Subjects:	Microglia iPSC ALSP CSF1R Inflammation Phagocytosis
Online Access:	https://doi.org/10.1186/s13024-024-00723-x

Similar Items

Minocycline protects against microgliopathy in a Csf1r haplo-insufficient mouse model of adult-onset leukoencephalopathy with axonal spheroids and pigmented glia (ALSP)
by: Xin Li, et al.
Published: (2023-05-01)

Deciphering glial contributions to CSF1R-related disorder via single-nuclear transcriptomic profiling: a case study
by: Jie Pan, et al.
Published: (2024-08-01)

Phenotypic characterization of a Csf1r haploinsufficient mouse model of adult-onset leukodystrophy with axonal spheroids and pigmented glia (ALSP)
by: Violeta Chitu, et al.
Published: (2015-02-01)

Trem2 Enhances Demyelination in the <i>Csf1r</i><sup>+/−</sup> Mouse Model of Leukoencephalopathy
by: Fabrizio Biundo, et al.
Published: (2023-07-01)

Hematopoietic Stem Cell Transplantation in <i>CSF1R</i>-Related Leukoencephalopathy: Retrospective Study on Predictors of Outcomes
by: Jarosław Dulski, et al.
Published: (2022-12-01)

Adult-onset diffuse leukoencephalopathy with axonal spheroids and pigmented glia presenting with acute stroke-like symptoms: A rare clinical scenario
by: Bela Purohit, MBBS, MD, DNB, FRCR (UK), EDiHNR, et al.
Published: (2020-10-01)

Case report: Treatment of advanced CSF1-receptor associated leukoencephalopathy with hematopoietic stem cell transplant
by: Caroline G. Bergner, et al.
Published: (2023-05-01)

Clinical presentation and diagnosis of adult-onset leukoencephalopathy with axonal spheroids and pigmented glia: a literature analysis of case studies
by: Spyros Papapetropoulos, et al.
Published: (2024-03-01)

Attenuated CSF‐1R signalling drives cerebrovascular pathology
by: Conor Delaney, et al.
Published: (2020-12-01)

Entropy, Said the Devil
by: Dorion Sagan
Published: (2023-11-01)

Gaia versus the Anthropocene: Untimely Thoughts on the Current Eco-Catastrophe
by: Dorion Sagan
Published: (2020-06-01)

Use of Polarized Light in the Diagnosis of “Fuzz” Granuloma in the Conjunctiva of a Child
by: Andrew R. Dorion, et al.
Published: (2021-04-01)

R. Kamtekar (éd.), Plato’s Euthyphro, Apology, and Crito. Critical essays
by: Louis-André Dorion
Published: (2006-06-01)

Socrate entremetteur
by: Louis-André Dorion
Published: (2009-11-01)

Le daimonion et la megalêgoria de Socrate dans l’Apologie de Xénophon
by: Louis-André Dorion
Published: (2008-04-01)

Platon, Proclus et l’Autarcie du Monde (Timée 33d)
by: Louis-André Dorion
Published: (2006-06-01)

COINING FOR A FACTION: COMPETING DEPICTIONS OF FOREIGN PEOPLES AND PLACES ON ROMAN COINAGE IN CIVIL WAR HISPANIA (RRC 468, RRC 469, RRC 470)
by: Ellen MacDougall
Published: (2020-07-01)

Kino transkulturowe (cz. 1)
by: David MacDougall
Published: (2004-12-01)

Kino transkulturowe (cz. 2)
by: David MacDougall
Published: (2005-06-01)

Walking the line: the boundaries between coaching and therapy
by: Lorna MacDougall
Published: (2024-06-01)

Predicting supervisor capacities to foster higher forms of learning through undergraduate medical student research
by: Margaret MacDougall
Published: (2014-02-01)

CelltypeR: A flow cytometry pipeline to characterize single cells from brain organoids
by: Rhalena A. Thomas, et al.
Published: (2024-09-01)

Novel regulators of heparan sulfate proteoglycans modulate cellular uptake of α-synuclein fibrils
by: Benoît Vanderperre, et al.
Published: (2025-10-01)

Generation of patient-derived pluripotent stem cell-lines and CRISPR modified isogenic controls with mutations in the Parkinson’s associated GBA gene
by: Carol X.-Q. Chen, et al.
Published: (2022-10-01)

Ataxin-3 and its E3 partners: Implications for Machado-Joseph disease
by: Thomas M Durcan, et al.
Published: (2013-05-01)

Angioimmunoblastic T-cell lymphoma presenting with an acute serologic Epstein-Barr virus profile
by: Timothy Beer, et al.
Published: (2015-06-01)

Limitations of the 1 % experiment as the benchmark idealized experiment for carbon cycle intercomparison in C<sup>4</sup>MIP
by: A. H. MacDougall
Published: (2019-02-01)

Estimated effect of the permafrost carbon feedback on the zero emissions commitment to climate change
by: A. H. MacDougall
Published: (2021-09-01)

Single cell RNAseq to identify subpopulations of glial progenitors in iPSC-derived oligodendroglial lineage cultures
by: Gabriela J. Blaszczyk, et al.
Published: (2025-04-01)

Observatoire de tourisme et de culture comme stratégie mise en valeur du développement régional
by: Edegar Luis Tomazzoni, et al.
Published: (2009-12-01)

Nitrogen addition enhances terrestrial phosphorous retention in grassland mesocosms
by: Ellen Esch, et al.
Published: (2023-04-01)

RIG-I-Like Receptor Signaling in Singleton-Merten Syndrome
by: Changming Lu, et al.
Published: (2017-09-01)

The missing bit in the middle: Implementation of the Nationals Health Services Standards for Papua New Guinea.
by: Elisabeth Schuele, et al.
Published: (2022-01-01)

Enacting Feminist Methodologies in Research Toward Reproductive Justice
by: Krista Johnston, et al.
Published: (2021-05-01)

Editorial: Origin and early evolution of amniotes
by: Jörg Fröbisch, et al.
Published: (2025-07-01)

Analysis of the microglia transcriptome across the human lifespan using single cell RNA sequencing
by: Moein Yaqubi, et al.
Published: (2023-05-01)

Quantifying the probability distribution function of the transient climate response to cumulative CO2 emissions
by: Lynsay Spafford, et al.
Published: (2020-01-01)

Projecting the release of carbon from permafrost soils using a perturbed parameter ensemble modelling approach
by: A. H. MacDougall, et al.
Published: (2016-04-01)

Enhancement of non‐CO2 radiative forcing via intensified carbon cycle feedbacks
by: Andrew H. MacDougall, et al.
Published: (2016-06-01)

Impact of deforestation on subsurface temperature profiles: implications for the borehole paleoclimate record
by: Andrew H MacDougall, et al.
Published: (2017-01-01)