Measurement of 2-methylthio Modifications in Mitochondrial Transfer RNAs by Reverse-transcription Quantitative PCR
2-Methylthio-N6-isopentenyladenosine (ms2i6A) is an evolutionally conserved posttranscriptional modification found at position 37 of four mammalian mitochondrial tRNAs, mt-tRNASer(UCN), mt-tRNATrp, mt-tRNAPhe and mt-tRNATyr. The ms2 modification in ms2i6A strengthens codon-anticodon interaction and...
| Published in: | Bio-Protocol |
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| Main Authors: | , |
| Format: | Article |
| Language: | English |
| Published: |
Bio-protocol LLC
2016-01-01
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| Online Access: | https://bio-protocol.org/en/bpdetail?id=1695&type=0 |