Dynamic interactions between the RNA chaperone Hfq, small regulatory RNAs, and mRNAs in live bacterial cells

RNA-binding proteins play myriad roles in regulating RNAs and RNA-mediated functions. In bacteria, the RNA chaperone Hfq is an important post-transcriptional gene regulator. Using live-cell super-resolution imaging, we can distinguish Hfq binding to different sizes of cellular RNAs. We demonstrate t...

Full description

Bibliographic Details
Published in:	eLife
Main Authors:	Seongjin Park, Karine Prévost, Emily M Heideman, Marie-Claude Carrier, Muhammad S Azam, Matthew A Reyer, Wei Liu, Eric Massé, Jingyi Fei
Format:	Article
Language:	English
Published:	eLife Sciences Publications Ltd 2021-02-01
Subjects:	hfq sRNA gene regulation single-particle tracking super-resolution imaging
Online Access:	https://elifesciences.org/articles/64207

Similar Items

Binding of the RNA Chaperone Hfq on Target mRNAs Promotes the Small RNA RyhB-Induced Degradation in <i>Escherichia coli</i>
by: David Lalaouna, et al.
Published: (2021-09-01)

The RNA Chaperone Hfq Participates in Persistence to Multiple Antibiotics in the Fish Pathogen <i>Yersinia ruckeri</i>
by: Iván L. Calderón, et al.
Published: (2021-06-01)

Recognition of the small regulatory RNA RydC by the bacterial Hfq protein
by: Daniela Dimastrogiovanni, et al.
Published: (2014-12-01)

Digitalizing heterologous gene expression in Gram‐negative bacteria with a portable ON/OFF module
by: Belén Calles, et al.
Published: (2019-12-01)

The RNA chaperone Hfq has a multifaceted role in Edwardsiella ictaluri
by: Ali Akgul, et al.
Published: (2024-07-01)

Editorial: Small non-coding RNAs in Gram negative bacteria
by: Bindu Subhadra, et al.
Published: (2024-05-01)

Pleiotropic regulatory function of the RNA chaperone Hfq in the Pseudomonas protegens FD6
by: Deyu Wen, et al.
Published: (2024-10-01)

Genome-wide profiling of Hfq-bound RNAs reveals the iron-responsive small RNA RusT in Caulobacter crescentus
by: Laura N. Vogt, et al.
Published: (2024-04-01)

Effect of small-interfering RNA (As1974) and HFq-binding proteins on resistance gene and host microRNA (miR-30C) expression in Pseudomonas aeruginosa-infected patients from Iraq
by: Bashaer Saad Jabbar Al fatlah, et al.
Published: (2023-01-01)

Hfq protein and GcvB small RNA tailoring of oppA target mRNA to levels allowing translation activation by MicF small RNA in Escherichia coli
by: Marie-Claude Carrier, et al.
Published: (2023-12-01)

Does the Hfq Protein Contribute to RNA Cargo Translocation into Bacterial Outer Membrane Vesicles?
by: Marisela Velez, et al.
Published: (2025-04-01)

Conditional Hfq Association with Small Noncoding RNAs in Pseudomonas aeruginosa Revealed through Comparative UV Cross-Linking Immunoprecipitation Followed by High-Throughput Sequencing
by: Kotaro Chihara, et al.
Published: (2019-12-01)

Hfq modulates system-wide regulatory networks controlling biocontrol property in Lysobacter enzymogenes
by: Xinyi Cheng, et al.
Published: (2026-03-01)

Models of Hfq interactions with small non-coding RNA in Gram-negative and Gram-positive bacteria
by: Derrick Watkins, et al.
Published: (2023-10-01)

Computational Insights into the Binding Mechanism of OxyS sRNA with Chaperone Protein Hfq
by: Mengxin Li, et al.
Published: (2021-11-01)

Hfq mutation confers increased cephalosporin resistance in Klebsiella pneumoniae
by: Li Xinran, et al.
Published: (2017-01-01)

The Escherichia coli Hfq protein: an unattended DNA-transactions regulator
by: Grzegorz M Cech, et al.
Published: (2016-07-01)

Apomorphine Targets the Pleiotropic Bacterial Regulator Hfq
by: Florian Turbant, et al.
Published: (2021-03-01)

The Denaturant- and Mutation-Induced Disassembly of <i>Pseudomonas aeruginosa</i> Hexameric Hfq Y55W Mutant
by: Victor Marchenkov, et al.
Published: (2022-06-01)

Integrated Hfq-interacting RNAome and transcriptomic analysis reveals complex regulatory networks of nitrogen fixation in root-associated Pseudomonas stutzeri A1501
by: Fanyang Lv, et al.
Published: (2024-06-01)

Multifaceted Interplay between Hfq and the Small RNA GssA in Pseudomonas aeruginosa
by: Silvia Santoro, et al.
Published: (2023-02-01)

Hfq balances energetic efficiency and antibiotic persistence in Acinetobacter baumannii
by: Abhiroop Sett, et al.
Published: (2025-09-01)

DNA Transactions in Bacteria and Membranes: A Place for the Hfq Protein?
by: Sylwia Bloch, et al.
Published: (2025-04-01)

Assessing the conservation and targets of putative sRNAs in Streptococcus pneumoniae
by: Matthew C. Eichelman, et al.
Published: (2025-08-01)

The Role of the Hfq Protein in Bacterial Resistance to Antibiotics: A Narrative Review
by: Sylwia Bloch, et al.
Published: (2025-02-01)

<i>Cis</i>- and <i>Trans</i>-Encoded Small Regulatory RNAs in <i>Bacillus subtilis</i>
by: Sabine Brantl, et al.
Published: (2021-09-01)

Plugging Small RNAs into the Network
by: Lars Barquist
Published: (2020-06-01)

Sibling rivalry: Related bacterial small RNAs and their redundant and non-redundant roles
by: Clayton eCaswell, et al.
Published: (2014-10-01)

An Hfq-dependent post-transcriptional mechanism fine tunes RecB expression in Escherichia coli
by: Irina Kalita, et al.
Published: (2025-08-01)

The Pleiotropic Phenotypes Caused by an <i>hfq</i> Null Mutation in <i>Vibrio harveyi</i>
by: Yiqin Deng, et al.
Published: (2023-11-01)

Role of the Bacterial Amyloid-like Hfq in Fluoroquinolone Fluxes
by: Florian Turbant, et al.
Published: (2023-12-01)

A Prophage-Encoded Small RNA Controls Metabolism and Cell Division in <named-content content-type="genus-species">Escherichia coli</named-content>
by: Divya Balasubramanian, et al.
Published: (2016-02-01)

Achieving robust synthetic tolerance in industrial E. coli through negative auto-regulation of a DsrA-Hfq module
by: Xiaofeng Yang, et al.
Published: (2024-09-01)

Molecular mechanism of enhanced ethanol tolerance associated with hfq overexpression in Zymomonas mobilis
by: Ying Tang, et al.
Published: (2022-12-01)

Hfq CLASH uncovers sRNA-target interaction networks linked to nutrient availability adaptation
by: Ira Alexandra Iosub, et al.
Published: (2020-05-01)

Bacterial RNA chaperones and chaperone-like riboregulators: behind the scenes of RNA-mediated regulation of cellular metabolism
by: Kai Katsuya-Gaviria, et al.
Published: (2022-12-01)

Prioritizing bona fide bacterial small RNAs with machine learning classifiers
by: Erik J.J. Eppenhof, et al.
Published: (2019-01-01)

Little reason to call them small noncoding RNAs
by: Silvia Ferrara, et al.
Published: (2023-06-01)

Small regulatory RNAs: from bench to bedside – a keystone symposia meeting report
by: Soo Mi Lee, et al.
Published: (2023-12-01)

The Leucine-Responsive Regulatory Protein Lrp Participates in Virulence Regulation Downstream of Small RNA ArcZ in <named-content content-type="genus-species">Erwinia amylovora</named-content>
by: Jeffrey K. Schachterle, et al.
Published: (2019-06-01)