TissUExM protocol for ultrastructure expansion microscopy of zebrafish larvae and mouse embryos

Summary: Expansion microscopy of millimeter-large mechanically heterogeneous tissues, such as whole vertebrate embryos, has been limited, particularly when combined with post-expansion immunofluorescence. Here, we present a protocol to perform ultrastructure expansion microscopy of whole vertebrate...

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Bibliographic Details

Published in:	STAR Protocols
Main Authors:	Emmanuelle Steib, Christina Vagena-Pantoula, Julien Vermot
Format:	Article
Language:	English
Published:	Elsevier 2023-06-01
Subjects:	Developmental Biology Microscopy Model Organisms Molecular Biology
Online Access:	http://www.sciencedirect.com/science/article/pii/S2666166723002150