Comparison of droplet digital PCR and quantitative real-time PCR in mcrA-based methanogen community analysis

Two different quantitative PCR platforms, droplet digital PCR (dd-PCR) and quantitative real-time PCR (qPCR), were compared in a mcrA-based methanogen community assay that quantifies ten methanogen sub-groups. Both technologies exhibited similar PCR efficiencies over at least four orders of magnitud...

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Bibliographic Details
Published in:Biotechnology Reports
Main Authors: Tae Gwan Kim, So-Yeon Jeong, Kyung-Suk Cho
Format: Article
Language:English
Published: Elsevier 2014-12-01
Subjects:
Droplet-digital PCR
Quantitative real-time PCR
Methanogen community
Anaerobic digester
mcrA
Online Access:http://www.sciencedirect.com/science/article/pii/S2215017X1400023X

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http://www.sciencedirect.com/science/article/pii/S2215017X1400023X

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