Protocol for generating human assembloids to investigate thalamocortical and corticothalamic synaptic transmission and plasticity

Summary: Human induced pluripotent stem cells (hiPSCs) can be used to generate assembloids that recreate thalamocortical circuitry displaying short-term and long-term synaptic plasticity. Here, we describe a protocol for differentiating hiPSCs into thalamic and cortical organoids and then fusing the...

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Bibliographic Details

Published in:	STAR Protocols
Main Authors:	Anjana Nityanandam, Mary H. Patton, Ildar T. Bayazitov, Kyle D. Newman, Kristen T. Thomas, Stanislav S. Zakharenko
Format:	Article
Language:	English
Published:	Elsevier 2025-03-01
Subjects:	Neuroscience Stem Cells Organoids
Online Access:	http://www.sciencedirect.com/science/article/pii/S266616672500036X