Flow cytometric protocol to characterize human memory B cells directed against SARS-CoV-2 spike protein antigens

Summary: Memory B cells (MBCs), part of the immune response elicited by infection or vaccination, can persist in lymphoid organs and peripheral blood and are capable of rapid reactivation upon secondary antigen exposure. Here, we describe a flow cytometric assay to identify antigen-specific MBCs fro...

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Bibliographic Details

Published in:	STAR Protocols
Main Authors:	Leonie M. Weskamm, Christine Dahlke, Marylyn M. Addo
Format:	Article
Language:	English
Published:	Elsevier 2022-12-01
Subjects:	Flow Cytometry/Mass Cytometry Immunology Microbiology
Online Access:	http://www.sciencedirect.com/science/article/pii/S2666166722007821