Generation of endogenously tagged E-cadherin cells using gene editing via non-homologous end joining

Summary: We provide a protocol using non-homologous end joining to integrate an oligonucleotide sequence of a fluorescence protein at the CDH1 locus encoding for the epithelial glycoprotein E-cadherin. We describe steps for implementing the CRISPR-Cas9-mediated knock-in procedure by transfecting a c...

Full description

Bibliographic Details

Published in:	STAR Protocols
Main Authors:	Natalie Rimmer, Ching-Yeu Liang, Ricardo Coelho, Monica Nunez Lopez, Francis Jacob
Format:	Article
Language:	English
Published:	Elsevier 2023-06-01
Subjects:	Cell Culture Flow Cytometry/Mass Cytometry Cell Separation/Fractionation Gene Expression CRISPR
Online Access:	http://www.sciencedirect.com/science/article/pii/S2666166723002721