Enhancing the nitric oxide inhibitory activity using a combination of plant essential oils and mixture design approach
The synergistic effects of essential oils (EOs) from three aromatic plant species, Foeniculum vulgare subsp. piperitum (C.Presl) Bég. (FV), Origanum heracleoticum L. (OH) and Lavandula austroapennina N.G.Passal., Tundis & Upson. (LA), were evaluated for their inhibitory properties on nitric...
| 出版年: | Heliyon |
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| 主要な著者: | , , , , , , , |
| フォーマット: | 論文 |
| 言語: | 英語 |
| 出版事項: |
Elsevier
2024-05-01
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| 主題: | |
| オンライン･アクセス: | http://www.sciencedirect.com/science/article/pii/S2405844024071111 |
| _version_ | 1850386381579223040 |
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| author | Mariangela Marrelli Michele De Luca Claudia-Crina Toma Fedora Grande Maria Antonietta Occhiuzzi Rosalba Caruso Filomena Conforti Giancarlo Statti |
| author_facet | Mariangela Marrelli Michele De Luca Claudia-Crina Toma Fedora Grande Maria Antonietta Occhiuzzi Rosalba Caruso Filomena Conforti Giancarlo Statti |
| author_sort | Mariangela Marrelli |
| collection | DOAJ |
| container_title | Heliyon |
| description | The synergistic effects of essential oils (EOs) from three aromatic plant species, Foeniculum vulgare subsp. piperitum (C.Presl) Bég. (FV), Origanum heracleoticum L. (OH) and Lavandula austroapennina N.G.Passal., Tundis & Upson. (LA), were evaluated for their inhibitory properties on nitric oxide production in RAW 264.7 macrophages stimulated with lipopolysaccharide (LPS). We utilized a Design of Experiments (DoE) methodology to optimize a formulation by combining three Essential Oils (EOs), while simultaneously taking into account two response variables, maximization of NO inhibition with minimum cytotoxicity. The optimal blend of components was predicted, and the statistical outcome's efficacy was experimentally verified. The combination corresponding to 87.7 % FV, 12.3 % LA and 0.0 % OH showed high inhibitory effect (76.3 %) with negligible cytotoxicity (4.5 %). This research provides new information on the interactions among fennel, oregano and lavender essential oils and shows how they can synergistically inhibit in vitro LPS-induced NO production. |
| format | Article |
| id | doaj-art-b3cdc330addf41cc9589eef1c5d3be4e |
| institution | Directory of Open Access Journals |
| issn | 2405-8440 |
| language | English |
| publishDate | 2024-05-01 |
| publisher | Elsevier |
| record_format | Article |
| spelling | doaj-art-b3cdc330addf41cc9589eef1c5d3be4e2025-08-19T22:55:27ZengElsevierHeliyon2405-84402024-05-011010e3108010.1016/j.heliyon.2024.e31080Enhancing the nitric oxide inhibitory activity using a combination of plant essential oils and mixture design approachMariangela Marrelli0Michele De Luca1Claudia-Crina Toma2Fedora Grande3Maria Antonietta Occhiuzzi4Rosalba Caruso5Filomena Conforti6Giancarlo Statti7Department of Pharmacy, Health and Nutritional Sciences, University of Calabria, 87036, Rende, Cosenza, ItalyDepartment of Pharmacy, Health and Nutritional Sciences, University of Calabria, 87036, Rende, Cosenza, Italy; Corresponding author.Pharmacology Department, Faculty of Pharmacy, Vasile Goldis Western University of Arad, 87 L. Rebreanu Str., 310045, Arad, RomaniaDepartment of Pharmacy, Health and Nutritional Sciences, University of Calabria, 87036, Rende, Cosenza, ItalyDepartment of Pharmacy, Health and Nutritional Sciences, University of Calabria, 87036, Rende, Cosenza, ItalyDepartment of Pharmacy, Health and Nutritional Sciences, University of Calabria, 87036, Rende, Cosenza, ItalyDepartment of Pharmacy, Health and Nutritional Sciences, University of Calabria, 87036, Rende, Cosenza, ItalyDepartment of Pharmacy, Health and Nutritional Sciences, University of Calabria, 87036, Rende, Cosenza, ItalyThe synergistic effects of essential oils (EOs) from three aromatic plant species, Foeniculum vulgare subsp. piperitum (C.Presl) Bég. (FV), Origanum heracleoticum L. (OH) and Lavandula austroapennina N.G.Passal., Tundis & Upson. (LA), were evaluated for their inhibitory properties on nitric oxide production in RAW 264.7 macrophages stimulated with lipopolysaccharide (LPS). We utilized a Design of Experiments (DoE) methodology to optimize a formulation by combining three Essential Oils (EOs), while simultaneously taking into account two response variables, maximization of NO inhibition with minimum cytotoxicity. The optimal blend of components was predicted, and the statistical outcome's efficacy was experimentally verified. The combination corresponding to 87.7 % FV, 12.3 % LA and 0.0 % OH showed high inhibitory effect (76.3 %) with negligible cytotoxicity (4.5 %). This research provides new information on the interactions among fennel, oregano and lavender essential oils and shows how they can synergistically inhibit in vitro LPS-induced NO production.http://www.sciencedirect.com/science/article/pii/S2405844024071111Essential oilsFennelLavenderMixture designNitric oxideOregano |
| spellingShingle | Mariangela Marrelli Michele De Luca Claudia-Crina Toma Fedora Grande Maria Antonietta Occhiuzzi Rosalba Caruso Filomena Conforti Giancarlo Statti Enhancing the nitric oxide inhibitory activity using a combination of plant essential oils and mixture design approach Essential oils Fennel Lavender Mixture design Nitric oxide Oregano |
| title | Enhancing the nitric oxide inhibitory activity using a combination of plant essential oils and mixture design approach |
| title_full | Enhancing the nitric oxide inhibitory activity using a combination of plant essential oils and mixture design approach |
| title_fullStr | Enhancing the nitric oxide inhibitory activity using a combination of plant essential oils and mixture design approach |
| title_full_unstemmed | Enhancing the nitric oxide inhibitory activity using a combination of plant essential oils and mixture design approach |
| title_short | Enhancing the nitric oxide inhibitory activity using a combination of plant essential oils and mixture design approach |
| title_sort | enhancing the nitric oxide inhibitory activity using a combination of plant essential oils and mixture design approach |
| topic | Essential oils Fennel Lavender Mixture design Nitric oxide Oregano |
| url | http://www.sciencedirect.com/science/article/pii/S2405844024071111 |
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