Protocol for tumor dissociation and fluorescence-activated cell sorting of human head and neck cancers

Summary: Tumors originating from the head and neck represent diverse histologies and are comprised of several cell types, including malignant cells, cancer-associated fibroblasts, endothelial cells, and immune cells. In this protocol, we describe a step-by-step approach for the dissociation of fresh...

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Bibliographic Details

Published in:	STAR Protocols
Main Authors:	Ogoegbunam Okolo, Victoria Yu, Samuel Flashner, Cecilia Martin, Hiroshi Nakagawa, Derrick T. Lin, Sidharth V. Puram, Anuraag S. Parikh
Format:	Article
Language:	English
Published:	Elsevier 2023-06-01
Subjects:	Cell Biology Cell culture Cell isolation Cell separation/fractionation Flow Cytometry/Mass Cytometry Single Cell
Online Access:	http://www.sciencedirect.com/science/article/pii/S2666166723002617