Center Degenerated Walking-Primer PCR: A Novel and Universal Genome-Walking Method

Enhancing the specificity and applicability of PCR-based genome-walking methods is highly desirable. A new and universal genome-walking tool, called center degenerated walking-primer PCR (CDWP-PCR), is presented in this study. CDWP-PCR involves adopting a center degenerated walking primer (cdWP) in...

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Bibliographic Details

Published in:	Current Issues in Molecular Biology
Main Authors:	Dandan Gao, Zhenkang Pan, Hao Pan, Yinwei Gu, Haixing Li
Format:	Article
Language:	English
Published:	MDPI AG 2025-08-01
Subjects:	genome-walking PCR normal walking primer center degenerated walking primer partial annealing gene-specific primer
Online Access:	https://www.mdpi.com/1467-3045/47/8/602