A Vaccinia-based system for directed evolution of GPCRs in mammalian cells

G protein-coupled receptors are a major class of drug targets. Here, the authors develop a method whereby their biophysical and functional properties can be altered through directed evolution in mammalian cells, leading to variants exhibiting features such as high stability and expression, or increa...

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Bibliographic Details

Published in:	Nature Communications
Main Authors:	Christoph Klenk, Maria Scrivens, Anina Niederer, Shuying Shi, Loretta Mueller, Elaine Gersz, Maurice Zauderer, Ernest S. Smith, Ralf Strohner, Andreas Plückthun
Format:	Article
Language:	English
Published:	Nature Portfolio 2023-03-01
Online Access:	https://doi.org/10.1038/s41467-023-37191-8