A Vaccinia-based system for directed evolution of GPCRs in mammalian cells

G protein-coupled receptors are a major class of drug targets. Here, the authors develop a method whereby their biophysical and functional properties can be altered through directed evolution in mammalian cells, leading to variants exhibiting features such as high stability and expression, or increa...

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Bibliographic Details
Published in:Nature Communications
Main Authors: Christoph Klenk, Maria Scrivens, Anina Niederer, Shuying Shi, Loretta Mueller, Elaine Gersz, Maurice Zauderer, Ernest S. Smith, Ralf Strohner, Andreas Plückthun
Format: Article
Language:English
Published: Nature Portfolio 2023-03-01
Online Access:https://doi.org/10.1038/s41467-023-37191-8
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Summary:G protein-coupled receptors are a major class of drug targets. Here, the authors develop a method whereby their biophysical and functional properties can be altered through directed evolution in mammalian cells, leading to variants exhibiting features such as high stability and expression, or increased allosteric coupling.
ISSN:2041-1723

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