A Vaccinia-based system for directed evolution of GPCRs in mammalian cells
G protein-coupled receptors are a major class of drug targets. Here, the authors develop a method whereby their biophysical and functional properties can be altered through directed evolution in mammalian cells, leading to variants exhibiting features such as high stability and expression, or increa...
| Published in: | Nature Communications |
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| Main Authors: | , , , , , , , , , |
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2023-03-01
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| Online Access: | https://doi.org/10.1038/s41467-023-37191-8 |
| _version_ | 1857120423440809984 |
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| author | Christoph Klenk Maria Scrivens Anina Niederer Shuying Shi Loretta Mueller Elaine Gersz Maurice Zauderer Ernest S. Smith Ralf Strohner Andreas Plückthun |
| author_facet | Christoph Klenk Maria Scrivens Anina Niederer Shuying Shi Loretta Mueller Elaine Gersz Maurice Zauderer Ernest S. Smith Ralf Strohner Andreas Plückthun |
| author_sort | Christoph Klenk |
| collection | DOAJ |
| container_title | Nature Communications |
| description | G protein-coupled receptors are a major class of drug targets. Here, the authors develop a method whereby their biophysical and functional properties can be altered through directed evolution in mammalian cells, leading to variants exhibiting features such as high stability and expression, or increased allosteric coupling. |
| format | Article |
| id | doaj-art-b76be3cffcda479f9ff2db12a5dfd09a |
| institution | Directory of Open Access Journals |
| issn | 2041-1723 |
| language | English |
| publishDate | 2023-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| spelling | doaj-art-b76be3cffcda479f9ff2db12a5dfd09a2025-08-19T19:09:20ZengNature PortfolioNature Communications2041-17232023-03-0114111410.1038/s41467-023-37191-8A Vaccinia-based system for directed evolution of GPCRs in mammalian cellsChristoph Klenk0Maria Scrivens1Anina Niederer2Shuying Shi3Loretta Mueller4Elaine Gersz5Maurice Zauderer6Ernest S. Smith7Ralf Strohner8Andreas Plückthun9Department of Biochemistry, University of ZurichVaccinex, Inc., 1895 Mt. Hope Avenue, RochesterDepartment of Biochemistry, University of ZurichVaccinex, Inc., 1895 Mt. Hope Avenue, RochesterVaccinex, Inc., 1895 Mt. Hope Avenue, RochesterVaccinex, Inc., 1895 Mt. Hope Avenue, RochesterVaccinex, Inc., 1895 Mt. Hope Avenue, RochesterVaccinex, Inc., 1895 Mt. Hope Avenue, RochesterMorphoSys AGDepartment of Biochemistry, University of ZurichG protein-coupled receptors are a major class of drug targets. Here, the authors develop a method whereby their biophysical and functional properties can be altered through directed evolution in mammalian cells, leading to variants exhibiting features such as high stability and expression, or increased allosteric coupling.https://doi.org/10.1038/s41467-023-37191-8 |
| spellingShingle | Christoph Klenk Maria Scrivens Anina Niederer Shuying Shi Loretta Mueller Elaine Gersz Maurice Zauderer Ernest S. Smith Ralf Strohner Andreas Plückthun A Vaccinia-based system for directed evolution of GPCRs in mammalian cells |
| title | A Vaccinia-based system for directed evolution of GPCRs in mammalian cells |
| title_full | A Vaccinia-based system for directed evolution of GPCRs in mammalian cells |
| title_fullStr | A Vaccinia-based system for directed evolution of GPCRs in mammalian cells |
| title_full_unstemmed | A Vaccinia-based system for directed evolution of GPCRs in mammalian cells |
| title_short | A Vaccinia-based system for directed evolution of GPCRs in mammalian cells |
| title_sort | vaccinia based system for directed evolution of gpcrs in mammalian cells |
| url | https://doi.org/10.1038/s41467-023-37191-8 |
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