Simultaneous engineering of natural killer cells for CAR transgenesis and CRISPR-Cas9 knockout using retroviral particles

Natural killer (NK) cells are potent cytotoxic innate lymphocytes that can be used for cancer immunotherapy. Since the balance of signals from activating and inhibitory receptors determines the activity of NK cells, their anti-tumor activity can be potentiated by overexpressing activating receptors...

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Bibliographic Details

Published in:	Molecular Therapy: Methods & Clinical Development
Main Authors:	Dong-Hyeon Jo, Shelby Kaczmarek, Oksu Shin, Lisheng Wang, Juthaporn Cowan, Scott McComb, Seung-Hwan Lee
Format:	Article
Language:	English
Published:	Elsevier 2023-06-01
Subjects:	natural killer cells genetic engineering CRISPR-Cas9 virus-like-particles retroviral particles chimeric antigen receptors
Online Access:	http://www.sciencedirect.com/science/article/pii/S2329050123000426