Reference genes selection and validation in yam by real-time quantitative polymerase chain reaction

Abstract Gene expression pattern analysis plays a crucial role in omics research, as it helps us understand the regulatory mechanisms of gene expression and the associated biological processes. Real-time quantitative polymerase chain reaction (q-PCR) is an efficient method for studying gene expressi...

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Bibliographic Details
Published in:Scientific Reports
Main Authors: Linan Xing, Yanfang Zhang, Yanping Xing, Mingran Ge, Huiqin Miao, Xiuwen Huo
Format: Article
Language:English
Published: Nature Portfolio 2025-06-01
Subjects:
Gene expression
Internal reference gene
Normalization
RT-qPCR
Yam
Online Access:https://doi.org/10.1038/s41598-025-92244-w

Internet

https://doi.org/10.1038/s41598-025-92244-w

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