Biochemical characterization, structure-guided mutagenesis, and application of a recombinant D-allulose 3-epimerase from Christensenellaceae bacterium for the biocatalytic production of D-allulose

D-Allulose has become a promising alternative sweetener due to its unique properties of low caloric content, moderate sweetness, and physiological effects. D-Allulose 3-epimerase (DAEase) is a promising enzyme for D-Allulose production. However, the low catalytic efficiency limited its large-scale i...

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Bibliographic Details

Published in:	Frontiers in Bioengineering and Biotechnology
Main Authors:	Lijun Guan, Ling Zhu, Kunlun Wang, Yang Gao, Jialei Li, Song Yan, Xindi Zhang, Nina Ji, Jing Fan, Ye Zhou, Xinmiao Yao, Bo Li
Format:	Article
Language:	English
Published:	Frontiers Media S.A. 2024-02-01
Subjects:	D-allulose D-allulose 3-epimerase bioconversion site-directed iteration mutagenesis apple juice
Online Access:	https://www.frontiersin.org/articles/10.3389/fbioe.2024.1365814/full