Transfer in SDS of biotinylated proteins from acrylamide gels to an avidin-coated membrane filter
Avidin was covalently linked to aldehyde-derivatized polyethersulfone membrane filters. These filters were used in Western blot analysis of proteins reacted with biotinylation reagents and electrophoresed in sodium dodecyl sulfate (SDS) on polyacrylamide gels. Electrophoretic transfer from the gels...
| Published in: | BioTechniques |
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| Main Authors: | , , , |
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2004-06-01
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| Online Access: | https://www.future-science.com/doi/10.2144/04366RR02 |