Germline ablation achieved via CRISPR/Cas9 targeting of NANOS3 in bovine zygotes

NANOS3 is expressed in migrating primordial germ cells (PGCs) to protect them from apoptosis, and it is known to be a critical factor for germline development of both sexes in several organisms. However, to date, live NANOS3 knockout (KO) cattle have not been reported, and the specific role of NANOS...

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Bibliographic Details

Published in:	Frontiers in Genome Editing
Main Authors:	Maci L. Mueller, Bret R. McNabb, Joseph R. Owen, Sadie L. Hennig, Alba V. Ledesma, Mitchell L. Angove, Alan J. Conley, Pablo J. Ross, Alison L. Van Eenennaam
Format:	Article
Language:	English
Published:	Frontiers Media S.A. 2023-11-01
Subjects:	germline ablation NANOS3 bovine embryo gene editing CRISPR/Cas9
Online Access:	https://www.frontiersin.org/articles/10.3389/fgeed.2023.1321243/full