Non-viral expression of chimeric antigen receptors with multiplex gene editing in primary T cells

Efficient engineering of T cells to express exogenous tumor-targeting receptors such as chimeric antigen receptors (CARs) or T-cell receptors (TCRs) is a key requirement of effective adoptive cell therapy for cancer. Genome editing technologies, such as CRISPR/Cas9, can further alter the functional...

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Bibliographic Details

Published in:	Frontiers in Bioengineering and Biotechnology
Main Authors:	Dan Cappabianca, Jingling Li, Yueting Zheng, Cac Tran, Kassandra Kasparek, Pedro Mendez, Ricky Thu, Travis Maures, Christian M. Capitini, Robert Deans, Krishanu Saha
Format:	Article
Language:	English
Published:	Frontiers Media S.A. 2024-05-01
Subjects:	multiplex gene editing CRISPR/Cas9 GD2 chimeric antigen receptor T cells PD-1 neuroblastoma
Online Access:	https://www.frontiersin.org/articles/10.3389/fbioe.2024.1379900/full