A simplified protocol for producing Taq DNA polymerase in biology laboratory

Background: Taq DNA polymerase is a very important enzyme for molecular biological studies such as DNA amplification and DNA sequencing by the PCR. It is a standard enzyme that is used in 90% of molecular biology labs today. The aim of this study was to produce Taq DNA polymerase enzyme in E. coli b...

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Bibliographic Details

Published in:	Research in Molecular Medicine
Main Authors:	Touraj Farazmandfar, Alireza Rafiei, Mohammad Bagher Hashemi-Sotehoh, Reza Valadan, Mohammad Alavi, Fatemeh Moradian
Format:	Article
Language:	English
Published:	Mazandaran University of Medical Sciences and Health Services 2013-09-01
Subjects:	Taq polymerase expression Purification
Online Access:	http://rmm.mazums.ac.ir/browse.php?a_code=A-10-26-9&slc_lang=en&sid=1