Rapid in-solution preparation of somatic and meiotic plant cell nuclei for high-quality 3D immunoFISH and immunoFISH-GISH

Abstract Background Though multicolour labelling methods allow the routine detection of a wide range of fluorescent (immuno)probe types in molecular cytogenetics, combined applications for the simultaneous in situ detection of proteins and nucleic acids are still sporadic in plant cell biology. A ma...

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Bibliographic Details

Published in:	Plant Methods
Main Authors:	Diána Makai, Edit Mihók, Dávid Polgári, András Cseh, Andrea Lenykó-Thegze, Adél Sepsi, László Sági
Format:	Article
Language:	English
Published:	BMC 2023-08-01
Subjects:	Barley Cereal Immunolabelling In situ hybridisation Interphase nuclei Wheat
Online Access:	https://doi.org/10.1186/s13007-023-01061-7