Protocol for decellularizing and delipidizing human pancreatic tissue into native hydrogel

Summary: We present a protocol for transforming human pancreatic tissue into a decellularized and delipidized extracellular matrix (ECM) for hydrogel/scaffold applications. We describe steps for decellularization and delipidization using sodium deoxycholate and sequential ethanol, acetone, and hexan...

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Bibliographic Details

Published in:	STAR Protocols
Main Authors:	Connie S. Chamberlain, Daniel M. Tremmel, Ayesha Palwasha Khan, Ellen Abad Santos, Deep Kapadia, Caterra Leavens, Katherine M. Gorski, Rawan Hamadeh, Austin K. Feeney, Jon Odorico, Sara D. Sackett
Format:	Article
Language:	English
Published:	Elsevier 2025-09-01
Subjects:	Organoids Tissue Engineering Biotechnology and bioengineering
Online Access:	http://www.sciencedirect.com/science/article/pii/S2666166725004034