Gene Splicing by Overlap Extension: Tailor-Made Genes Using the Polymerase Chain Reaction
Gene Splicing by Overlap Extension or “gene SOEing” is a PCR-based method of recombining DNA sequences without reliance on restriction sites and of directly generating mutated DNA fragments in vitro. By modifying the sequences incorporated into the 5′-ends of the primers, any pair of polymerase chai...
| Published in: | BioTechniques |
|---|---|
| Main Authors: | , , , |
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2013-03-01
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| Online Access: | https://www.future-science.com/doi/10.2144/000114017 |