Visualizing Filamentous Actin in Chlamydomonas reinhardtii

This protocol aims to visualize the filamentous actin network in Chlamydomonas reinhardtii. We improved fixed-cell labeling conditions using the F-actin probe, phalloidin. We created a Chlamydomonas-optimized protocol by halving the phalloidin incubation time, electing for optimal fixation condition...

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Bibliographic Details
Published in:Bio-Protocol
Main Authors: Evan Craig, Prachee Avasthi
Format: Article
Language:English
Published: Bio-protocol LLC 2019-06-01
Online Access:https://bio-protocol.org/en/bpdetail?id=3274&type=0