Purification of hetero-oligomeric protein variants using a modified tandem affinity purification approach

Summary: Here, we describe a protocol for artificially generating hetero-oligomeric protein complexes from the homo-oligomers using a sequential denaturation-renaturation strategy, followed by a modified affinity chromatography protocol used for their purification. This protocol enables one to obtai...

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Bibliographic Details

Published in:	STAR Protocols
Main Authors:	Aasna L. Parui, Kakoli Bose
Format:	Article
Language:	English
Published:	Elsevier 2023-03-01
Subjects:	Biophysics Protein Biochemistry Protein expression and purification
Online Access:	http://www.sciencedirect.com/science/article/pii/S2666166722009157