Ratiometric imaging of calcium during ischemia-reperfusion injury in isolated mouse hearts using Fura-2

<p>Abstract</p> <p>Background</p> <p>We present an easily implementable method for measuring Fura-2 fluorescence from isolated mouse hearts using a commercially available switching light source and CCD camera. After calibration, it provides a good estimate of intracellu...

Full description

Bibliographic Details

Published in:	BioMedical Engineering OnLine
Main Authors:	Venkataraman Raghav, Holcomb Mark R, Harder Rene, Knollmann Björn C, Baudenbacher Franz
Format:	Article
Language:	English
Published:	BMC 2012-07-01
Subjects:	Optical Mapping Langendorff Mouse Heart Whole-Heart Fura-2 Ischemia-Reperfusion Conduction Velocity Reperfusion Injury LAD ligation Ratiometric Calcium Imaging
Online Access:	http://www.biomedical-engineering-online.com/content/11/1/39