Ratiometric imaging of calcium during ischemia-reperfusion injury in isolated mouse hearts using Fura-2

• Published in: BioMedical Engineering OnLine
• Main Authors: Venkataraman Raghav, Holcomb Mark R, Harder Rene, Knollmann Björn C, Baudenbacher Franz
• Format: Article
• Language: English
• Published: BMC 2012-07-01
• Subjects: Optical Mapping; Langendorff Mouse Heart; Whole-Heart Fura-2; Ischemia-Reperfusion; Conduction Velocity; Reperfusion Injury; LAD ligation; Ratiometric Calcium Imaging
• Online Access: http://www.biomedical-engineering-online.com/content/11/1/39

<p>Abstract</p> <p>Background</p> <p>We present an easily implementable method for measuring Fura-2 fluorescence from isolated mouse hearts using a commercially available switching light source and CCD camera. After calibration, it provides a good estimate of intracellular [Ca²⁺] with both high spatial and temporal resolutions, permitting study of changes in dispersion of diastolic [Ca²⁺], Ca²⁺ transient dynamics, and conduction velocities in mouse hearts. In a proof-of-principle study, we imaged isolated Langendorff-perfused mouse hearts with reversible regional myocardial infarctions.</p> <p>Methods</p> <p>Isolated mouse hearts were perfused in the Landendorff-mode and loaded with Fura-2. Hearts were then paced rapidly and subjected to 15 minutes of regional ischemia by ligation of the left anterior descending coronary artery, following which the ligation was removed to allow reperfusion for 15 minutes. Fura-2 fluorescence was recorded at regular intervals using a high-speed CCD camera. The two wavelengths of excitation light were interleaved at a rate of 1 KHz with a computer controlled switching light source to illuminate the heart.</p> <p>Results</p> <p>Fura-2 produced consistent Ca²⁺ transients from different hearts. Ligating the coronary artery rapidly generated a well defined region with a dramatic rise in diastolic Ca²⁺ without a significant change in transient amplitude; Ca²⁺ handling normalized during reperfusion. Conduction velocity was reduced by around 50% during ischemia, and did not recover significantly when monitored for 15 minutes following reperfusion.</p> <p>Conclusions</p> <p>Our method of imaging Fura-2 from isolated whole hearts is capable of detecting pathological changes in intracellular Ca²⁺ levels in cardiac tissue. The persistent change in the conduction velocities indicates that changes to tissue connectivity rather than altered intracellular Ca²⁺ handling may be underlying the electrical instabilities commonly seen in patients following a myocardial infarction.</p>