Electroporation of CRISPR/Cas9 Targeting Neurogenin 3 (NGN3) in Porcine Embryos and Its Effects on Mosaicism and Off-Target Effects by Next Generation Sequencing (NGS)

Introduction: CRISPR/Cas9-mediated editing of embryos through microinjection is limited in efficiency because of high rates of mosaicism and off-target mutations. Electroporation has been proposed as an easier and faster procedure compared to single embryo injection. Methods: Using a synthetic guide...

Full description

Bibliographic Details

Published in:	Re:GEN Open
Main Authors:	Insung Park, Sergio Navarro-Serna, Raquel M. Pinho, Trish Berger, Elizabeth A. Maga, Joaqu?n Gadea, Seung K. Kim, Pablo J. Ross
Format:	Article
Language:	English
Published:	Mary Ann Liebert 2024-01-01
Subjects:	Pig embryos CRISPR/Cas9 electroporation mosaicism off-target effects
Online Access:	https://www.liebertpub.com/doi/full/10.1089/REGEN.2023.0021