Trophectoderm Transcriptome Analysis in LIN28 Knockdown Ovine Conceptuses Suggests Diverse Roles of the LIN28-let-7 Axis in Placental and Fetal Development
The proper conceptus elongation in ruminants is critical for the successful placentation and establishment of pregnancy. We have previously shown that the trophectoderm-specific knockdown of LIN28A/B in day 9 ovine blastocysts resulted in increased let-7 miRNAs and reduced conceptus elongation at da...
| 出版年: | Cells |
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| 主要な著者: | , , , , , , |
| フォーマット: | 論文 |
| 言語: | 英語 |
| 出版事項: |
MDPI AG
2022-04-01
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| 主題: | |
| オンライン･アクセス: | https://www.mdpi.com/2073-4409/11/7/1234 |
| _version_ | 1851077386583408640 |
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| author | Asghar Ali Muhammad A. Iqbal Muhammad W. Abbas Gerrit J. Bouma Russell V. Anthony Thomas E. Spencer Quinton A. Winger |
| author_facet | Asghar Ali Muhammad A. Iqbal Muhammad W. Abbas Gerrit J. Bouma Russell V. Anthony Thomas E. Spencer Quinton A. Winger |
| author_sort | Asghar Ali |
| collection | DOAJ |
| container_title | Cells |
| description | The proper conceptus elongation in ruminants is critical for the successful placentation and establishment of pregnancy. We have previously shown that the trophectoderm-specific knockdown of LIN28A/B in day 9 ovine blastocysts resulted in increased let-7 miRNAs and reduced conceptus elongation at day 16 of gestation. In this current study, by transcriptome analysis of LIN28A knockdown (AKD) or LIN28B knockdown (BKD) trophectoderm (TE), we explored the downstream target genes of the LIN28-let-7 axis and their roles in the placental and fetal development. We identified 449 differentially expressed genes (DEGs) in AKD TE and 1214 DEGs in BKD TE compared to non-targeting control (NTC). Our analysis further revealed that 210 downregulated genes in AKD TE and 562 downregulated genes in BKD TE were the potential targets of let-7 miRNAs. Moreover, 16 downregulated genes in AKD TE and 57 downregulated and 7 upregulated genes in BKD TE were transcription factors. The DEGs in AKD and BKD TE showed enrichment in the biological processes and pathways critical for placental development and function, and fetal development and growth. The results of this study suggest the potential roles of the LIN28-let-7 axis in placental and fetal development beyond its involvement in trophoblast proliferation and conceptus elongation. |
| format | Article |
| id | doaj-art-cb2bbf63b3df4b1cbdc08565aafcd978 |
| institution | Directory of Open Access Journals |
| issn | 2073-4409 |
| language | English |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| spelling | doaj-art-cb2bbf63b3df4b1cbdc08565aafcd9782025-08-19T22:33:08ZengMDPI AGCells2073-44092022-04-01117123410.3390/cells11071234Trophectoderm Transcriptome Analysis in LIN28 Knockdown Ovine Conceptuses Suggests Diverse Roles of the LIN28-let-7 Axis in Placental and Fetal DevelopmentAsghar Ali0Muhammad A. Iqbal1Muhammad W. Abbas2Gerrit J. Bouma3Russell V. Anthony4Thomas E. Spencer5Quinton A. Winger6Animal Reproduction and Biotechnology Laboratory, Department of Biomedical Sciences, 1683 Campus Delivery, Colorado State University, Fort Collins, CO 80523, USAInstitute of Genome Biology, Research Institute for Farm Animal Biology, 18196 Dummerstorf, GermanySchool of Interdisciplinary Engineering and Sciences, National University of Science and Technology, Islamabad 44000, PakistanAnimal Reproduction and Biotechnology Laboratory, Department of Biomedical Sciences, 1683 Campus Delivery, Colorado State University, Fort Collins, CO 80523, USAAnimal Reproduction and Biotechnology Laboratory, Department of Biomedical Sciences, 1683 Campus Delivery, Colorado State University, Fort Collins, CO 80523, USAAnimal Science Research Center, College of Agriculture, Food and Natural Resources, University of Missouri, Columbia, MO 65211, USAAnimal Reproduction and Biotechnology Laboratory, Department of Biomedical Sciences, 1683 Campus Delivery, Colorado State University, Fort Collins, CO 80523, USAThe proper conceptus elongation in ruminants is critical for the successful placentation and establishment of pregnancy. We have previously shown that the trophectoderm-specific knockdown of LIN28A/B in day 9 ovine blastocysts resulted in increased let-7 miRNAs and reduced conceptus elongation at day 16 of gestation. In this current study, by transcriptome analysis of LIN28A knockdown (AKD) or LIN28B knockdown (BKD) trophectoderm (TE), we explored the downstream target genes of the LIN28-let-7 axis and their roles in the placental and fetal development. We identified 449 differentially expressed genes (DEGs) in AKD TE and 1214 DEGs in BKD TE compared to non-targeting control (NTC). Our analysis further revealed that 210 downregulated genes in AKD TE and 562 downregulated genes in BKD TE were the potential targets of let-7 miRNAs. Moreover, 16 downregulated genes in AKD TE and 57 downregulated and 7 upregulated genes in BKD TE were transcription factors. The DEGs in AKD and BKD TE showed enrichment in the biological processes and pathways critical for placental development and function, and fetal development and growth. The results of this study suggest the potential roles of the LIN28-let-7 axis in placental and fetal development beyond its involvement in trophoblast proliferation and conceptus elongation.https://www.mdpi.com/2073-4409/11/7/1234microRNAsblastocystRNA sequencingLIN28ALIN28Bfetal growth |
| spellingShingle | Asghar Ali Muhammad A. Iqbal Muhammad W. Abbas Gerrit J. Bouma Russell V. Anthony Thomas E. Spencer Quinton A. Winger Trophectoderm Transcriptome Analysis in LIN28 Knockdown Ovine Conceptuses Suggests Diverse Roles of the LIN28-let-7 Axis in Placental and Fetal Development microRNAs blastocyst RNA sequencing LIN28A LIN28B fetal growth |
| title | Trophectoderm Transcriptome Analysis in LIN28 Knockdown Ovine Conceptuses Suggests Diverse Roles of the LIN28-let-7 Axis in Placental and Fetal Development |
| title_full | Trophectoderm Transcriptome Analysis in LIN28 Knockdown Ovine Conceptuses Suggests Diverse Roles of the LIN28-let-7 Axis in Placental and Fetal Development |
| title_fullStr | Trophectoderm Transcriptome Analysis in LIN28 Knockdown Ovine Conceptuses Suggests Diverse Roles of the LIN28-let-7 Axis in Placental and Fetal Development |
| title_full_unstemmed | Trophectoderm Transcriptome Analysis in LIN28 Knockdown Ovine Conceptuses Suggests Diverse Roles of the LIN28-let-7 Axis in Placental and Fetal Development |
| title_short | Trophectoderm Transcriptome Analysis in LIN28 Knockdown Ovine Conceptuses Suggests Diverse Roles of the LIN28-let-7 Axis in Placental and Fetal Development |
| title_sort | trophectoderm transcriptome analysis in lin28 knockdown ovine conceptuses suggests diverse roles of the lin28 let 7 axis in placental and fetal development |
| topic | microRNAs blastocyst RNA sequencing LIN28A LIN28B fetal growth |
| url | https://www.mdpi.com/2073-4409/11/7/1234 |
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