Protocol to characterize mouse dural mast cells by flow cytometry and immunofluorescence

Summary: Mast cells, which constitute tissue-resident immune cells, are distributed in the dural meninges. Here, we provide procedural guidelines for investigating mouse dural mast cells using two techniques. First, we outline the procedures for dural tissue dissection, single-cell isolation, and su...

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Bibliographic Details

Published in:	STAR Protocols
Main Authors:	Chih-Chung Jerry Lin, Nader Jaafar, Rudolph E. Tanzi
Format:	Article
Language:	English
Published:	Elsevier 2024-12-01
Subjects:	Cell Biology Cell isolation Single Cell Flow Cytometry Immunology Microscopy
Online Access:	http://www.sciencedirect.com/science/article/pii/S266616672400529X