Analysis of mouse intestinal organoid culture with conditioned media isolated from mucosal enteric glial cells

Summary: This protocol describes the isolation and culture of 3D intestinal crypt organoids with stromal niche cells. We show a murine organoid culture system that utilizes conditioned media isolated from primary, mucosal enteric glial cell culture. We describe three assays of analyzing this organoi...

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Bibliographic Details

Published in:	STAR Protocols
Main Authors:	Meryem B. Baghdadi, Tae-Hee Kim
Format:	Article
Language:	English
Published:	Elsevier 2022-06-01
Subjects:	Cell Biology Cell culture Cell isolation Flow Cytometry/Mass Cytometry Microbiology Molecular Biology
Online Access:	http://www.sciencedirect.com/science/article/pii/S2666166722002313