Development and validation of an HILIC/MS/MS method for determination of nusinersen in rabbit plasma

A hydrophilic interaction liquid chromatography tandem mass spectrometry (HILIC/MS/MS) method was developed and validated for the quantitative analysis of the fully phosphorothioate modified oligonucleotide nusinersen. HILIC/MS/MS method is more robust and compatible with mass spectrometry than ion...

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Bibliographic Details

Published in:	Heliyon
Main Authors:	Xiao Zhang, Chunjie Sha, Wei Zhang, Fengjuan Zhao, Mingli Zhu, Guangyi Leng, Wanhui Liu
Format:	Article
Language:	English
Published:	Elsevier 2024-05-01
Subjects:	Nusinersen HILIC/MS/MS Phosphorothioate modification Pharmacokinetics Quantitation
Online Access:	http://www.sciencedirect.com/science/article/pii/S240584402407244X