Sequencing accuracy and systematic errors of nanopore direct RNA sequencing

Abstract Background Direct RNA sequencing (dRNA-seq) on the Oxford Nanopore Technologies (ONT) platforms can produce reads covering up to full-length gene transcripts, while containing decipherable information about RNA base modifications and poly-A tail lengths. Although many published studies have...

書誌詳細
出版年:	BMC Genomics
主要な著者:	Wang Liu-Wei, Wiep van der Toorn, Patrick Bohn, Martin Hölzer, Redmond P. Smyth, Max von Kleist
フォーマット:	論文
言語:	英語
出版事項:	BMC 2024-05-01
主題:	Nanopore sequencing Direct RNA sequencing Transcriptomics Epitranscriptomics Sequencing errors Sequencing accuracy
オンライン･アクセス:	https://doi.org/10.1186/s12864-024-10440-w

類似資料

Nanopore-Based Direct RNA-Sequencing Reveals a High-Resolution Transcriptional Landscape of Porcine Reproductive and Respiratory Syndrome Virus
著者:: Riteng Zhang, 等
出版事項: (2021-12-01)

Sequoia: an interactive visual analytics platform for interpretation and feature extraction from nanopore sequencing datasets
著者:: Ratanond Koonchanok, 等
出版事項: (2021-07-01)

The promising role of nanopore sequencing in cancer diagnostics and treatment
著者:: Xinming Su, 等
出版事項: (2025-04-01)

Barcode-free multiplex plasmid sequencing using Bayesian analysis and nanopore sequencing
著者:: Masaaki Uematsu, 等
出版事項: (2025-04-01)

An open-source nanopore-only sequencing workflow for analysis of clonal outbreaks delivers short-read level accuracy
著者:: Nick Vereecke, 等
出版事項: (2025-08-01)

Species-specific basecallers improve actual accuracy of nanopore sequencing in plants
著者:: Scott Ferguson, 等
出版事項: (2022-12-01)

One step forward for nanopore protein sequencing
著者:: Ziyi Li, 等
出版事項: (2024-03-01)

Identifying and correcting repeat-calling errors in nanopore sequencing of telomeres
著者:: Kar-Tong Tan, 等
出版事項: (2022-08-01)

Basecalling Using Joint Raw and Event Nanopore Data Sequence-to-Sequence Processing
著者:: Adam Napieralski, 等
出版事項: (2022-03-01)

Explore of nanopore sequencing technology in ambiguities of HLA genotyping
著者:: CHEN Nanying, 等
出版事項: (2025-03-01)

Accurate and reproducible whole-genome genotyping for bacterial genomic surveillance with Nanopore sequencing data
著者:: K. Prior, 等
出版事項: (2025-07-01)

Nanopore Technology and Its Applications in Gene Sequencing
著者:: Bo Lin, 等
出版事項: (2021-06-01)

In-Depth Temporal Transcriptome Profiling of an Alphaherpesvirus Using Nanopore Sequencing
著者:: Dóra Tombácz, 等
出版事項: (2022-06-01)

Applying nanopore sequencing in the etiological diagnosis of bloodstream infection
著者:: Yiqun Liao, 等
出版事項: (2025-02-01)

An Evaluation of Avian Influenza Virus Whole-Genome Sequencing Approaches Using Nanopore Technology
著者:: Hon S. Ip, 等
出版事項: (2023-02-01)

Automated evaluation of multiple sequence alignment methods to handle third generation sequencing errors
著者:: Coralie Rohmer, 等
出版事項: (2024-09-01)

Whole-Genome Sequencing of Human Enteroviruses from Clinical Samples by Nanopore Direct RNA Sequencing
著者:: Carole Grädel, 等
出版事項: (2020-07-01)

Evaluation of Nanopore Sequencing for Diagnosing Pulmonary Tuberculosis Using Negative Smear Clinical Specimens
著者:: Yu G, 等
出版事項: (2024-02-01)

Nanopore sequencing for smear-negative pulmonary tuberculosis—a multicentre prospective study in China
著者:: Xiaojing Yan, 等
出版事項: (2024-06-01)

Nanopore Metagenomics Sequencing for Rapid Diagnosis and Characterization of Lily Viruses
著者:: Hyo-Jeong Lee, 等
出版事項: (2022-10-01)

Profiling the polyadenylated transcriptome of extracellular vesicles with long-read nanopore sequencing
著者:: Juan-Carlos A. Padilla, 等
出版事項: (2023-09-01)

HycDemux: a hybrid unsupervised approach for accurate barcoded sample demultiplexing in nanopore sequencing
著者:: Renmin Han, 等
出版事項: (2023-10-01)

Imputation strategies for genomic prediction using nanopore sequencing
著者:: H. J. Lamb, 等
出版事項: (2023-12-01)

Analysis of Wheat Virome in Korea Using Illumina and Oxford Nanopore Sequencing Platforms
著者:: Hyo-Jeong Lee, 等
出版事項: (2023-06-01)

Nanopore targeted sequencing identifies pathogens in patients with postoperative endophthalmitis
著者:: Xin-Lei Hao, 等
出版事項: (2025-08-01)

Nanopore sequencing of protozoa: Decoding biological information on a string of biochemical molecules into human-readable signals
著者:: Branden Hunter, 等
出版事項: (2025-01-01)

Nanopore 16S-Full Length and ITS Sequencing for Microbiota Identification in Intra-Abdominal Infections
著者:: Jian-Jhou Liao, 等
出版事項: (2025-09-01)

Flesh ID: Nanopore Sequencing Combined with Offline BLAST Search for the Identification of Meat Source
著者:: Jonas Kissenkötter, 等
出版事項: (2020-10-01)

Prediction of transcript isoforms in 19 chicken tissues by Oxford Nanopore long-read sequencing
著者:: Dailu Guan, 等
出版事項: (2022-10-01)

Differentiation of Capripox Viruses by Nanopore Sequencing
著者:: Kamal H. Eltom, 等
出版事項: (2021-04-01)

Effects of error, chimera, bias, and GC content on the accuracy of amplicon sequencing
著者:: Yujia Qin, 等
出版事項: (2023-12-01)

Direct Nanopore Sequencing of Human Cytomegalovirus Genomes from High-Viral-Load Clinical Samples
著者:: Kathy K. Li, 等
出版事項: (2023-05-01)

Evaluation of Commercial RNA Extraction Protocols for Avian Influenza Virus Using Nanopore Metagenomic Sequencing
著者:: Maria Chaves, 等
出版事項: (2024-09-01)

RAPiD: a rapid and accurate plant pathogen identification pipeline for on-site nanopore sequencing
著者:: Stephen Knobloch, 等
出版事項: (2024-09-01)

A framework for real-time monitoring, analysis and adaptive sampling of viral amplicon nanopore sequencing
著者:: Rory Munro, 等
出版事項: (2023-03-01)

Evaluating the potential and limitations of nanopore adaptive sampling for targeted transcriptome sequencing
著者:: Nicole DeBruyne, 等
出版事項: (2025-10-01)

Targeted Whole Genome Sequencing (TWG-Seq) of Cucumber Green Mottle Mosaic Virus Using Tiled Amplicon Multiplex PCR and Nanopore Sequencing
著者:: Joanne Mackie, 等
出版事項: (2022-10-01)

Nanopore direct RNA sequencing maps the complexity of Arabidopsis mRNA processing and m6A modification
著者:: Matthew T Parker, 等
出版事項: (2020-01-01)

Cattle connection: molecular epidemiology of BVDV outbreaks via rapid nanopore whole-genome sequencing of clinical samples
著者:: Jacqueline King, 等
出版事項: (2021-07-01)

Kastor: a reference-based comparative approach for assessment and correction of gene-fragmenting errors in long-read assemblies of small genomes
著者:: Janet S.H. Lorv, 等
出版事項: (2025-04-01)