The balance between gyrase and topoisomerase I activities determines levels of supercoiling, nucleoid compaction, and viability in bacteria

Two enzymes are responsible for maintaining supercoiling in the human pathogen Streptococcus pneumoniae, gyrase (GyrA2GyrB2) and topoisomerase I. To attain diverse levels of topoisomerase I (TopoI, encoded by topA), two isogenic strains derived from wild-type strain R6 were constructed: PZntopA, car...

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Bibliographic Details

Published in:	Frontiers in Microbiology
Main Authors:	Míriam García-López, Diego Megias, María-José Ferrándiz, Adela G. de la Campa
Format:	Article
Language:	English
Published:	Frontiers Media S.A. 2023-01-01
Subjects:	DNA supercoiling DNA topoisomerase I DNA gyrase regulation of supercoiling supercoiling homeostasis
Online Access:	https://www.frontiersin.org/articles/10.3389/fmicb.2022.1094692/full