Mass spectrometry and split luciferase complementation assays reveal the MecA protein interactome of Streptococcus mutans

ABSTRACTMecA is a highly conserved adaptor protein encoded by prokaryotes from the Bacillota phylum. MecA mutants exhibit similar pleiotropic defects in a variety of organisms, although most of these phenotypes currently lack a mechanistic basis. MecA mediates ClpCP-dependent proteolysis of its subs...

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Bibliographic Details

Published in:	Microbiology Spectrum
Main Authors:	Hua Qin, David Anderson, Zhengzhong Zou, Dustin Higashi, Christina Borland, Jens Kreth, Justin Merritt
Format:	Article
Language:	English
Published:	American Society for Microbiology 2024-02-01
Subjects:	Streptococcus mutans MecA interactome split luciferase complementation assays mass spectrometry protein binding affinity
Online Access:	https://journals.asm.org/doi/10.1128/spectrum.03691-23