Design and Direct Assembly of Synthesized Uracil-containing Non-clonal DNA Fragments into Vectors by USERTM Cloning

This protocol describes how to order and directly assemble uracil-containing non-clonal DNA fragments by uracil excision based cloning (USER cloning). The protocol was generated with the goal of making synthesized non-clonal DNA fragments directly compatible with USERTM cloning. The protocol is high...

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Bibliographic Details

Published in:	Bio-Protocol
Main Authors:	Morten Jørgensen, Nikolai Wulff, Majse Nafisi, Deyang Xu, Cuiwei Wang, Sophie Lambertz, Zeinu Belew, Hussam Nour-Eldin
Format:	Article
Language:	English
Published:	Bio-protocol LLC 2017-11-01
Online Access:	https://bio-protocol.org/en/bpdetail?id=2615&type=0