Design and Direct Assembly of Synthesized Uracil-containing Non-clonal DNA Fragments into Vectors by USERTM Cloning
This protocol describes how to order and directly assemble uracil-containing non-clonal DNA fragments by uracil excision based cloning (USER cloning). The protocol was generated with the goal of making synthesized non-clonal DNA fragments directly compatible with USERTM cloning. The protocol is high...
| Published in: | Bio-Protocol |
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| Main Authors: | , , , , , , , |
| Format: | Article |
| Language: | English |
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Bio-protocol LLC
2017-11-01
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| Online Access: | https://bio-protocol.org/en/bpdetail?id=2615&type=0 |
| _version_ | 1850147072490078208 |
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| author | Morten Jørgensen Nikolai Wulff Majse Nafisi Deyang Xu Cuiwei Wang Sophie Lambertz Zeinu Belew Hussam Nour-Eldin |
| author_facet | Morten Jørgensen Nikolai Wulff Majse Nafisi Deyang Xu Cuiwei Wang Sophie Lambertz Zeinu Belew Hussam Nour-Eldin |
| author_sort | Morten Jørgensen |
| collection | DOAJ |
| container_title | Bio-Protocol |
| description | This protocol describes how to order and directly assemble uracil-containing non-clonal DNA fragments by uracil excision based cloning (USER cloning). The protocol was generated with the goal of making synthesized non-clonal DNA fragments directly compatible with USERTM cloning. The protocol is highly efficient and would be compatible with uracil-containing non-clonal DNA fragments obtained from any synthesizing company. The protocol drastically reduces time and handling between receiving the synthesized DNA fragments and transforming with vector and DNA fragment(s). |
| format | Article |
| id | doaj-art-d2a4b831c44f4fdbabab3edc2cc70fe7 |
| institution | Directory of Open Access Journals |
| issn | 2331-8325 |
| language | English |
| publishDate | 2017-11-01 |
| publisher | Bio-protocol LLC |
| record_format | Article |
| spelling | doaj-art-d2a4b831c44f4fdbabab3edc2cc70fe72025-08-19T23:46:57ZengBio-protocol LLCBio-Protocol2331-83252017-11-0172210.21769/BioProtoc.2615Design and Direct Assembly of Synthesized Uracil-containing Non-clonal DNA Fragments into Vectors by USERTM CloningMorten Jørgensen0Nikolai Wulff1Majse Nafisi2Deyang Xu3Cuiwei Wang4Sophie Lambertz5Zeinu Belew6Hussam Nour-Eldin7University of Würzburg, Institute for Molecular Plant Physiology and Biophysics, Würzburg, GermanyDynaMo Center, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Frederiksberg C, DenmarkCopenhagen Plant Science Center, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Frederiksberg C, DenmarkCopenhagen Plant Science Center, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Frederiksberg C, DenmarkPlant Biochemistry Laboratory, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Frederiksberg C, DenmarkDynaMo Center, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Frederiksberg C, DenmarkCopenhagen Plant Science Center, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Frederiksberg C, DenmarkDynaMo Center, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Frederiksberg C, DenmarkCopenhagen Plant Science Center, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Frederiksberg C, DenmarkDynaMo Center, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Frederiksberg C, DenmarkCopenhagen Plant Science Center, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Frederiksberg C, DenmarkDynaMo Center, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Frederiksberg C, DenmarkCopenhagen Plant Science Center, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Frederiksberg C, DenmarkDynaMo Center, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Frederiksberg C, DenmarkCopenhagen Plant Science Center, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Frederiksberg C, DenmarkThis protocol describes how to order and directly assemble uracil-containing non-clonal DNA fragments by uracil excision based cloning (USER cloning). The protocol was generated with the goal of making synthesized non-clonal DNA fragments directly compatible with USERTM cloning. The protocol is highly efficient and would be compatible with uracil-containing non-clonal DNA fragments obtained from any synthesizing company. The protocol drastically reduces time and handling between receiving the synthesized DNA fragments and transforming with vector and DNA fragment(s). https://bio-protocol.org/en/bpdetail?id=2615&type=0 |
| spellingShingle | Morten Jørgensen Nikolai Wulff Majse Nafisi Deyang Xu Cuiwei Wang Sophie Lambertz Zeinu Belew Hussam Nour-Eldin Design and Direct Assembly of Synthesized Uracil-containing Non-clonal DNA Fragments into Vectors by USERTM Cloning |
| title | Design and Direct Assembly of Synthesized Uracil-containing Non-clonal DNA Fragments into Vectors by USERTM Cloning |
| title_full | Design and Direct Assembly of Synthesized Uracil-containing Non-clonal DNA Fragments into Vectors by USERTM Cloning |
| title_fullStr | Design and Direct Assembly of Synthesized Uracil-containing Non-clonal DNA Fragments into Vectors by USERTM Cloning |
| title_full_unstemmed | Design and Direct Assembly of Synthesized Uracil-containing Non-clonal DNA Fragments into Vectors by USERTM Cloning |
| title_short | Design and Direct Assembly of Synthesized Uracil-containing Non-clonal DNA Fragments into Vectors by USERTM Cloning |
| title_sort | design and direct assembly of synthesized uracil containing non clonal dna fragments into vectors by usertm cloning |
| url | https://bio-protocol.org/en/bpdetail?id=2615&type=0 |
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