rprimer: an R/bioconductor package for design of degenerate oligos for sequence variable viruses

Abstract Background This paper presents a new R/Bioconductor package, rprimer, for design of degenerate oligos and PCR assays for sequence variable viruses. A multiple DNA sequence alignment is used as input data, while the outputs consist of comprehensive tables (data frames) and dashboard-like plo...

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Bibliographic Details

Published in:	BMC Bioinformatics
Main Authors:	Sofia Persson, Christina Larsson, Magnus Simonsson, Patrik Ellström
Format:	Article
Language:	English
Published:	BMC 2022-06-01
Subjects:	Primer design Degenerate primers PCR RT-PCR qPCR Digital PCR
Online Access:	https://doi.org/10.1186/s12859-022-04781-0