Efficient induction and rapid identification of haploid grains in tetraploid wheat by editing genes TtMTL and pyramiding anthocyanin markers

Doubled haploid (DH) technology provides an effective way to generate homozygous genetic and breeding materials over a short period of time. We produced three types of homozygous TtMTL gene-edited mutants (mtl-a, mtl-b, and mtl-ab) by CRISPR/Cas9 in durum wheat. PCR restriction enzymes and sequencin...

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Bibliographic Details

Published in:	Frontiers in Plant Science
Main Authors:	Yanan Chang, Huali Tang, Surong Wang, Xi Li, Peipei Huang, Jiahui Zhang, Ke Wang, Yueming Yan, Xingguo Ye
Format:	Article
Language:	English
Published:	Frontiers Media S.A. 2024-03-01
Subjects:	durum wheat TtMTL genes CRISPR/Cas9 haploid induction purple embryo
Online Access:	https://www.frontiersin.org/articles/10.3389/fpls.2024.1346364/full