Protocol for the visualization of pRps6-positive cells in larval zebrafish brains using whole-mount immunofluorescence and light-sheet microscopy

Summary: Due to their small size and transparency, larval zebrafish are a useful model for whole-brain imaging. Here, we present a protocol for the visualization of phosphorylated Rps6, a marker of mechanistic target of rapamycin complex 1 (mTORC1) activity, in the zebrafish brains at 5 days post fe...

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Bibliographic Details

Published in:	STAR Protocols
Main Authors:	Olga Doszyn, Tomasz Dulski, Justyna Zmorzynska
Format:	Article
Language:	English
Published:	Elsevier 2025-03-01
Subjects:	developmental biology microscopy model organisms neuroscience
Online Access:	http://www.sciencedirect.com/science/article/pii/S2666166724007524