A robust and reliable methodology to perform GECI-based multi-time point neuronal calcium imaging within mixed cultures of human iPSC-derived cortical neurons

IntroductionHuman induced pluripotent stem cells (iPSCs), with their ability to generate human neural cells (astrocytes and neurons) from patients, hold great promise for understanding the pathophysiology of major neuropsychiatric diseases such as schizophrenia and bipolar disorders, which includes...

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發表在:	Frontiers in Neuroscience
Main Authors:	Niraj Patel, Vincent Ouellet, François Paquet-Mercier, Nizar Chetoui, Erik Bélanger, Marie-Eve Paquet, Antoine G. Godin, Pierre Marquet
格式:	Article
語言:	英语
出版:	Frontiers Media S.A. 2023-09-01
主題:	human iPSCs (induced pluripotent stem cells) neurodifferentiation GECI (genetically encoded Ca2+ indicator) human synapsin1 (hSyn) promoter CAG promoter calcium imaging
在線閱讀:	https://www.frontiersin.org/articles/10.3389/fnins.2023.1247397/full