Standardization of Sequencing Coverage Depth in NGS: Recommendation for Detection of Clonal and Subclonal Mutations in Cancer Diagnostics

The insufficient standardization of diagnostic next-generation sequencing (NGS) still limits its implementation in clinical practice, with the correct detection of mutations at low variant allele frequencies (VAF) facing particular challenges. We address here the standardization of sequencing covera...

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Bibliographic Details

Published in:	Frontiers in Oncology
Main Authors:	Anna Petrackova, Michal Vasinek, Lenka Sedlarikova, Tereza Dyskova, Petra Schneiderova, Tomas Novosad, Tomas Papajik, Eva Kriegova
Format:	Article
Language:	English
Published:	Frontiers Media S.A. 2019-09-01
Subjects:	next-generation sequencing variant allele frequency (VAF) coverage depth calculator sequencing error small subclones TP53 gene
Online Access:	https://www.frontiersin.org/article/10.3389/fonc.2019.00851/full